Pipette with one mark GOST 29169. Pipettes with one mark

Approved Decree of the Committee for Standardization and Metrology of the USSR of December 16, 1991 N 1952

State standard of the USSR GOST 29169-91 (ISO 648-77)

"LABORATORY GLASS WARE. PIPETTE WITH ONE MARK"

laboratory glassware. One-mark pipettes

1. Purpose and scope

This International Standard covers a range of single mark booties for general laboratory use. General requirements - according to GOST 29044 (ISO 384).

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. Links

GOST 29044 (ISO 384) "Laboratory glassware. Principles of construction and design of measuring glassware".

3. Basic units of measurement

3.1. Capacity unit

A cubic centimeter (cm 3) is taken as a unit of capacity, it is allowed to use the name milliliter (ml).

Note. Thorn milliliter (ml) is usually used as a special name for a cubic centimeter (cm 3) in accordance with the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20°C.

Note. When using pipettes in tropical conditions, it is allowed to take not 20 ° C, but 27 ° C as the standard water temperature, as recommended by GOST 29044 (ISO 384).

4. Volume measurement accuracy

Two classes of accuracy are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. Range of capacities

Single-mark pipettes must be manufactured to nominal capacity; 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 ml are made without a reservoir, 1 ml - with and without a reservoir, 2 ml - with a reservoir for the 1st class and with and without a reservoir - for the 2nd class.

All other sizes are for reservoir pipettes.

If necessary, pipettes of all sizes can be made with safety reservoirs above the graduation mark.

Note. Where necessary, pipettes of other nominal capacities may be made provided they conform to the essential requirements of this standard.

6. Determination of capacity

The capacity of a single-mark pipette is defined as the volume of water, expressed in milliliters, at 20° C., as described below.

The pipette in the vertical position should be filled with liquid a few millimeters above the graduation mark, and the meniscus drop should then be adjusted to the mark by one of the methods described below:

a) the meniscus is set so that the plane of the upper edge of the calibration mark is horizontal and tangent to the lower point of the meniscus, the observer's gaze is in the same plane;

Then, any droplets adhering to the spout tip at the moment of contact of the spout tip with the surface of the glass vessel are removed.

After that, the liquid must be drained into another, slightly inclined glass vessel so that the drain tip is in contact with the inner wall of the vessel, but without moving them relative to each other during the drain and the waiting period.

The pipette is released until the meniscus drops to the drain hole. To ensure that the drain is complete, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which no waiting time is set.

Note. The waiting period (approximately 3 s) is set in order to determine the time to drain the liquid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the draining edge of the pipette before removing the pipette from the receiving vessel.

If the pipette is set to a waiting time of 15 seconds, then this time must be maintained before removing the pipette from the receiving vessel.

Note. Class 1 pipettes must be:

a) without a specified waiting time, when this time is approximately equal to 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must be without a specified waiting time as defined in subparagraph a.

7. Limits of permissible error by volume

7.1. The limits of permissible error of the rated capacity should not exceed the values \u200b\u200bspecified in Table. 1.

Table 1

Rated capacity	Limit of error
Rated capacity

_____________________________

* For pipettes used for the needs of the national economy

7.2. The ratio of the nominal capacity and the maximum permissible error of the nominal capacity are given in Annex A, and the ratio of the maximum permissible error of the nominal capacity and the meniscus diameter is given in Annex B.

8. Construction

8.1. Material

Pipettes with a single mark should be made of glass with adequate chemical and thermal resistance and, as far as possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.6 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and pipettes of the 2nd class with a nominal capacity of 2 ml may be shown in Fig. 1 design or may have a reservoir as indicated for large sizes.

All large size pipettes consist of a reservoir with suction and discharge tubes. These parts must be straight and coaxial.

The shape of the pipettes should correspond to that shown in Fig. 1.

"Damn. 1. Pipette Shape"

If a safety reservoir is provided, it is approximately spherical and is located between the graduation mark and the top of the pipette.

It is allowed for the needs of the national economy to manufacture pipettes with one mark of the following designs:

1 - straight lines (Fig. 2);

"Dev. 2. Variants of execution of pipettes with one mark"

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with extension and spare tank (Fig. 2).

An example of a symbol for a pipette used for the needs of the national economy, execution 1, 2nd class, with a nominal capacity of 50 ml:

Pipette 1 - 2 - 50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Table. 2 and 3.

table 2

Dimensions in mm

Size name	Nominal capacity, ml
Size name
Total length, no more than:
straight pipettes
pipettes with reservoir
Suction tube length*(1), not less than
Drain tube length*(1), not less than
Inner diameter at the graduated mark*(2), no more
Drain tube outer diameter*(1) (limit deviation ±1 mm)
Tank diameter*(1), no more
Straight pipette tube diameter, no more

_____________________________

*(1) Reservoir pipettes only.

*(2) For straight pipettes, maximum inside diameter; for reservoir pipettes, the maximum inside diameter of the suction tube.

*(3) Calculated minimum distance from the calibration mark to the end of the drain hole, equal to 120 mm (see table. 3).

*(4) For pipettes used for the needs of the national economy.

The outer diameter of the discharge tube and the diameter of the straight pipette tube for pipettes used for the needs of the national economy are recommended.

Table 3

Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less than
Distance from the calibration mark to the top of the tank, not less than
Distance from the graduation mark to the cone of the drain hole of straight pipettes, not less than
Pipette wall thickness, not less than:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more than
Distance from the top of the pipette to the top of the safety reservoir, not less than

Note. Allowed for pipettes used for the needs of the national economy:

1) expansion wall thickness for pipettes with a reservoir - not less than 0.4 mm;

2) the value of the diameter of the safety tank - take as a reference size.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that might interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or smooth polished with a slight outward bevel.

8.5. Storage tank

The main part of the reservoir is cylindrical, except for pipettes with a nominal capacity of 1 and 2 ml, which may have an oval reservoir if necessary. The two ends of the tank should gradually pass into the suction and drain pipes. Pipette connections should be tapered to facilitate draining.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp constrictions at the drain hole.

8.6.1. The end of the drain tip must be processed by one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of breakage in use, but should not cause constriction as specified in 8.6.1 or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to fall from the graduation mark to the drain hole.

The drain time is determined with the pipette in a vertical position, and the receiving vessel should be slightly tilted so that the tip of the drain tube is in contact with the inner wall of the vessel, but without moving relative to each other.

Thus, the drain time should be within the limits indicated in Table. 4.

Table 4

Drain time, s

Accuracy class	For pipettes with a nominal capacity, ml
Accuracy class
1st class without defined waiting time:


1st class with a waiting time of 15 s:


2nd grade;


Maximum allowable difference between actual and indicated drain time*(1)

_____________________________

*(1) If the drain time is indicated on the pipette, in accordance with clause 10.2, then the actual and indicated time values \u200b\u200bshould not differ by more than the value indicated in table. 4,

*(2) For pipettes used for the needs of the national economy.

9. Graduation mark

The graduation mark should be a clear, uniform, indelible line that completely encircles the tube. A break in the mark is allowed, not exceeding 10% of the circumference. The line must be in a plane perpendicular to the axis of the pipette. The line thickness should not exceed 0.4 mm, which is provided for by the requirements of clause 8.2 of GOST 29044 (ISO 384).

The dimensions that determine the position of the graduation mark on the pipette are shown in Table. 2.

10. Lettering

10.1. All pipettes must bear the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is graduated (see note to 3.1);

Note. So, where the standard temperature is 27°C, call this value, instead of 20°C;

c) the letter "O", indicating that the pipette is designed to drain the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

e) waiting time, if set, in the form "0 + 15 s".

10.2. Class 1 pipettes intended for official verification or certification should bear an additional marking, which should preferably be applied to other class 1 pipettes and, if necessary, to class 2 pipettes;

a) the designation of this standard;

b) drain time in seconds.

10.3. Permissible error limit of nominal capacity in accordance with Table. 1 can be applied to all pipettes, eg ±...ml.

11. Graduation marks, numbers and inscriptions

11.1. All numbers and designations must be of such dimensions and shapes as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and designations must be clearly visible and indelible.

12. Color coding

If color coding is used, then it must be carried out in accordance with the requirements of the regulatory and technical documentation approved in the prescribed manner.

Annex A

The ratio between the capacity and the maximum permissible error for pipettes of the 1st class in accordance with the requirements of paragraph 4.4 of GOST 29044 (ISO 384)

Annex B

The ratio between the limits of permissible error and the diameter of the meniscus for pipettes of the 1st class in accordance with the requirements of paragraph 4.7 of GOST 29044 (ISO 384)

Annex 1

Additional requirements reflecting the needs of the national economy

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of pipettes (clause 8.3) should be checked with a universal measuring tool in accordance with GOST 166 and GOST 427.

13.3. The drain time of pipettes should be checked according to clause 8.7 of this standard.

14. Packing, transportation and storage

14.1. Pipettes are packed in such a way that their safety during transportation and storage is ensured.

14.2. Pipettes packed according to clause 14.1 are placed in a shipping container, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

GOST 29169-91 (ISO 648-77)

INTERSTATE STANDARD

LABORATORY GLASS WARE

PIPETTE WITH SINGLE MARK

Official edition

Standartinform

UDC 542.3:006.354

INTERSTATE

Group P66

STANDARD

Laboratory glassware GOST

PIPETTE WITH ONE MARK

laboratory glassware. One-mark pipettes

MKS 71.040.20 OKP 43 2133

Date of introduction 01.01.94

1. PURPOSE AND SCOPE

This International Standard covers a range of single-mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

GOST 29044-91 (ISO 384-78) Laboratory glassware. Principles of arrangement and design of measuring utensils

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

A cubic centimeter (cm 3) is taken as a unit of capacity, it is allowed to use the name milliliter (ml).

Note. The term milliliter (mL) is usually used as a special name for the cubic centimeter (cm 3 ) according to the International System of Units (SI).

3.2. Standard temperature

The standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained, is 20 °C.

Note. When using pipettes in tropical conditions, it is allowed to take not 20 ° C, but 27 ° C as the standard water temperature, as recommended by GOST 29044.

4. VOLUME MEASUREMENT ACCURACY

Two classes of accuracy are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for reservoir pipettes.

Official edition

Reprint prohibited

If necessary, pipettes of all sizes can be made with safety reservoirs above the graduation mark.

Note. Where necessary, pipettes of other nominal capacities may be made provided they conform to the essential requirements of this standard.

The capacity of a single-marked pipette is defined as the volume of water, expressed in milliliters, at 20 °C, as described below.

a) the meniscus is set so that the plane of the upper edge of the calibration mark is horizontal and tangent to the lower point of the meniscus, the observer's gaze is in the same plane;

Then, any droplets adhering to the spout tip at the moment of contact of the spout tip with the surface of the glass vessel are removed.

If the pipette is set to a waiting time of 15 seconds, then this time must be maintained before removing the pipette from the receiving vessel.

Note. Class 1 pipettes must be:

a) without a specified waiting time, when this time is approximately equal to 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must be without a specified waiting time as defined in subparagraph a.

6. DETERMINATION OF CAPACITY

Limit of error

Rated capacity

±0.01

±0.02

±0.1

±0.01

±0.02

±0.1

±0.2

7.2. The relation between the nominal capacity and the maximum permissible error of the nominal capacity is given in Appendix A, and the relation between the maximum permissible error of the nominal capacity and the meniscus diameter is given in Appendix B.

8. CONSTRUCTION

8.1. Material

Pipettes with a single mark should be made of glass with adequate chemical and thermal resistance and, as far as possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and pipettes of the 2nd class with a nominal capacity of 2 ml may be shown in Fig. 1 design or may have a reservoir as indicated for large sizes.

All large size pipettes consist of a reservoir with suction and discharge tubes. These parts must be straight and coaxial.

The shape of the pipettes should correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical and is located between the graduation mark and the top of the pipette.

It is allowed for the needs of the national economy to manufacture pipettes with one mark of the following designs:

1 - straight lines (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with extension and spare tank (Fig. 2).

Version 1 Version 1a Version 2 Version 2a

An example of a symbol for a pipette used for the needs of the national economy, execution 1, 2nd class, with a nominal capacity of 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Table. 2 and 3.

Dimensions in mm

table 2

Size name	Nominal capacity, ml
Size name
Total length, no more than: straight pipettes pipettes with reservoir
Suction tube length 1), not less than
Drain tube length 1), not less than
Inner diameter at the calibration mark 2), no more
Drain tube outer diameter 1) (limit deviation ±1 mm)
Tank diameter 1), no more
Straight pipette tube diameter, no more

^ Reservoir pipettes only.

^ For straight pipettes - maximum inner diameter; for reservoir pipettes, the maximum inside diameter of the suction tube.

^ Estimated minimum distance from the calibration mark to the end of the drain hole, equal to 120 mm (see table. 3).

^ For pipettes used for the needs of the national economy.

The outer diameter of the discharge tube and the diameter of the straight pipette tube for pipettes used for the needs of the national economy are recommended.

Table 3

Size name

Meaning

Distance of the graduation mark from the top of the pipette, at least Distance from the graduation mark to the top of the tank, at least Distance from the graduation mark to the drain cone of straight pipettes, at least

Pipette wall thickness, not less than: with a reservoir with a nominal capacity of 1 and 2 ml of other pipettes

Safety tank diameter

Distance from the top of the pipette to the bottom of the safety reservoir, not more than Distance from the top of the pipette to the top of the safety reservoir, not less than

Note. Allowed for pipettes used for the needs of the national economy:

1) expansion wall thickness for pipettes with a reservoir - not less than 0.4 mm;

2) the value of the diameter of the safety tank - take as a reference size.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that might interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or smooth polished with a slight outward bevel.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp constrictions at the drain hole.

8.6.2. The end of the drain tip must be processed by one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of breakage in use, but should not cause constriction as specified in and. 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to fall from the graduation mark to the drain hole.

Thus, the drain time should be within the limits indicated in Table. 4.

Table 4

Accuracy class		Drain time, s, for pipettes with a nominal capacity,
Accuracy class
1st class without fixed waiting time: at least

1st class with a waiting time of 15 s: not less than

2nd class: no less

The maximum allowable difference between the actual and indicated drain time

^ If the drain time is indicated on the pipette in accordance with and. 10.2, then the actual and indicated time values should not differ by more than the value indicated in Table. 4.

^ For pipettes used for the needs of the national economy.

9. CALIBRATION MARK

The dimensions that determine the position of the graduation mark on the pipette are shown in Table. 2.

10. SIGNS

10.1. All pipettes must bear the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is graduated (see note to 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

c) the letter "O", indicating that the pipette is designed to drain the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

f) waiting time, if set, in the form "0+15 s".

10.2. Class 1 pipettes intended for official verification or certification should be marked with an additional marking, preferably applied to other class 1 pipettes, and, if necessary, to class 2 pipettes:

a) the designation of this standard;

b) drain time in seconds.

10.3. Permissible error limit of nominal capacity in accordance with Table. 1 can be applied to all pipettes, e.g. ± ... ml.

11. MARKINGS, NUMBERS AND SIGNS

11.1. All numbers and designations must be of such dimensions and shapes as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and designations must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, then it must be carried out in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATION BETWEEN THE CAPACITY AND THE LIMIT OF PERMISSIBLE ERROR FOR PIPETTES OF THE 1ST CLASS IN ACCORDANCE WITH THE REQUIREMENTS of clause 4.4 of GOST 29044

APPENDIX B

RELATIONSHIP BETWEEN THE PERMISSIBLE ERRORS AND THE MENISCA DIAMETER FOR CLASS 1 PIPETTE IN ACCORDANCE WITH THE REQUIREMENTS of paragraph 4.7 of GOST 29044

Permissible error limit for Hgo class, ± µl

Meniscus diameter, mm

ANNEX 1

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of pipettes (i. 8.3) should be checked with a universal measuring tool in accordance with GOST 166 and GOST 427.

13.3. The drain time of the pipettes should be checked by and. 8.7 of this standard.

14. Packing, transportation and storage

14.1. Pipettes are packed in such a way that their safety during transportation and storage is ensured.

14.2. Pipettes packed by and. 14.1 are placed in a shipping container, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Elinsky Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND INTRODUCED BY Decree of the Committee for Standardization and Metrology of the USSR of December 16, 1991 No. 1952

This standard has been prepared by direct application of the international standard ISO 648-77 “Laboratory glassware. Pipettes with one mark "with additional requirements reflecting the needs of the national economy

3. INSTEAD OF GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIONS AND TECHNICAL DOCUMENTS

5. REPUBLICATION. March 2008

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GOST 29169-91 (ISO 648-77)

INTERSTATE STANDARD

LABORATORY GLASS WARE

PIPETTE WITH SINGLE MARK

Official edition

UDC 542.3:006.354 Group P66

INTERSTATE STANDARD

Laboratory glassware GOST

29169-91 SINGLE MARK PIPETTE

(ISO 648-77)

GABBOGA Zama One-mark arene

ISS TEN. 20 OKP 3+3 2115

Date of introduction 01.01.94

1. PURPOSE AND SCOPE

This standard is distributed in pairs of fully marked pipettes for general laboratory use. General requirements according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements. reflecting the needs of the national economy are given in Appendix 1.

GOST 29044 EEZISO 334 78) Laboratory glass promise. Principles of arrangement and design of a dimensional promise

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit The cubic centimeter (cm) is taken as the capacity unit. It is not acceptable to use the designation milliliter (mL).

Note. The term mialiliter (mot is usually used as a special name for the bark centimeter em ‘tv in accordance with the International System of Units CIT

3.2. Standard temperature Standard temperature, i.e. temperature. at which the nominal volume (nominal capacity) is drained. is 20″.

Note. When using a beer stick in tropical conditions, doplecasten for CLAM APTI KD TEM peragura, take no No. C. at 27 °C. as recommended by GOST 291044.

4. VOLUME MEASUREMENT ACCURACY

Set the leva of the accuracy class: I - for higher measurement accuracy: 2nd for less accurate measurements.

5. RANGE OF CAPACITIES

Single-marked pipettes must be manufactured with a nominal capacity of: 0.5. [; 2:9:K:20; 25; M: None 200 ml.

For the needs of the national economy, it is also allowed to produce pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 mA are made without a reservoir. [ml with and without reservoir. 2ml with 1st class bark reservoir and with and without reservoir for 2nd class.

All other sizes are for reservoir pipettes.

Official publication Reprint denied

\ Publishing house of standards. 1992 © Stanlartinfurm. 208

S. 2 GOST 29169-91

If necessary, pipettes of all sizes can also be supplied with safety reservoirs at the greyscale mark.

Note If necessary, pipettes of other nominal capacities should be used when pouring. they will correspond to the basic requirements of this mill.

6. DETERMINATION OF CAPACITY

The pillette in the upright position should be filled to a few millimeters above the graduation mark. and the burning of the meniscus should then be adjusted to the mark with one of the metols. described below:

a) the meniscus is set like this. so that the plane of the upper edge of the graduation mark is horizontal and tangent to the lower point of the meniscus. the observer's wobbling is in the same plane:

6) the meniscus is set like this. so that the plane of the mark nengra is horizontal tangent to the lower point of the meniscus. The observer's glare is directed upwards to this plane. in case of scrap, at the same time there should be forks pouring and filling parts of the mark. simultaneously converging in the lower part of the meniscus.

Then remove all drops. adhering to the tip at the moment of contact of the drain cup with the surface of the glass vessel.

After that, the vein cannot be drained into another. a slightly bowed glass vessel. so that the drain tip is in contact with the inner wall of the vessel. but without moving them relative to each other during the drain and the waiting period.

Inletka has been released since then. until the menek drops below the drain hole. For gogo to be sure. that the drain is over. come to life Zs. before removing the pipette from the vessel. This applies to pipettes. for which no waiting time is set.

Note. Pernel recovery approximately No. c) is used in determining the time to drain the liquid. There is no need to adhere exactly to this time. With aedu to be sure. chi msnisk reached the glorious edge of DUCT RI before taking out the post of Igor's sauce.

If the bark of the pipette is set, the waiting time is 15 sec. yu lo time should be borne before. how to remove the pill from the receiving vessel.

Note. 1st class pilots must be

i) Sun: specified time of day. when this time is approximately equal to Ze.

$) with a wait time of 15 s.

2nd class kicks must be of limited waiting time as defined by I a.

7. VOLUME ERROR LIMITS

7.1. Permissible capacity limits of rated capacity must not exceed the value. indicated in the table. 1.

Table 1

Prezel error tolerance

Rated capacity

2nd grade

5 10.43 10 21.04 1.77 #0413 m:0.06 28 20.06 89 #0. 1

14) 21.1% 24H) 2.2

on Aa RE LOK, barking OTP COMOCTOH HUG VOUT Bab

GOST 29169-91 S. 3

7.2. The ratio of the nominal capacity and the margin of error of the nominal capacity is given in Appendix A. and the ratio of the margin of error of the memorial capacity and the diameter of the meniscus is given in Appendix B.

8. CONSTRUCTION

$.1. Material

Fully marked pyrettes must be made of glass with appropriate chemical and thermal resistance. whenever possible. free from visible defects and internal stress.

Pipettes of nominal capacity (0.5 ml) consist of a straight tube with a drain hole at the lower end.

Booties nominal capacity | ml and pipettes of the 2nd class with a nominal capacity of - - 2 ml may have a figure shown. | construction or may have a reservoir. as indicated for large sizes.

All large size pipettes consist of a reservoir with a weighing tube and a drain tube. These parts must be straight and coaxial.

The shape of the pipettes should correspond to that shown in Fig. 1.

If a safety tank is provided. it has an approximately spherical shape and is located between the graduation mark and the top of the bootie.

It is allowed for the needs of the national economy and the tinting of booties with one mark of the following design:

| straight lines (Fig. 2);

1a straight lines with a spare tank (Fig. 2);

2 with extension (Fig. 29

2 with extension and spare tank (Fig. 2).

Sweating ( Version ba Version 2 No.

S. $ GOST 29169-91

nN An example of a pipette convention. used by the needs of the national economy. performance |. 2nd grade. nominal capacity 50 ml:

Pipette 2 ZOTOST 29 9 8.3. Dimensions The dimensions of the pellets must correspond to those indicated in Table 2 and 3.

Taolinia 2

Rizmers nmm

Rated capacity. m1

Olina iia, He Gade. spicy lines and booties with a reservoir

OSU Vessel length of the drain pipe", not less than 160 (Tu Length of the drain pipe", not less than 160 240 4 4.8 &.5 Outer lismegr of the drain pipe" approx. mm! 6.8 9 Tank diameter! not more than 16 449

He gotee diameter

11. Only 44th tank

` Dania pipettes - maximum diameter. for tanks with a tank - the maximum inner diameter of the non-causing tube.

“The estimated minimum distance from the trawling mark to the horse drain hole, equal to 1209 mm, is. those 3).

4) Dalia booties. used ala potreonost and folk

Table 3

Name lasmeya Meaning

Distance of the graduation mark og the upper part of the pipette, not less

marks 1 of the top of the tank, not chenss

10 Distances og the trawl mark ao of the discharge cone of straight pipettes. not less than fu Goinina of the wall of the zine, not less than: not more than Tu Distance from the upper part of the pines to the upper part of the safety tank. m not less

Note. The barking of pinetaks, the barking of the people's potreony 1 is allowed! wall thickness of expansion ala pieestek with tanks - not less than 0.4 mm.

21 value of the diameter of the safety reservoir - take as the size of the bark reference.

GOST 29169-91 S. 5

$.4. The upper part of the bootie must be perpendicular to the axis of the bootie and free from any defects. which can interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or polished with a slight bevel on the outside.

$.5. Storage tank

The main part of the reservoir is nilinaric. with the exception of pipettes with a nominal capacity Ti of 2 ml. who may have. if it is necessary. oval tank. The two ends of the tank should gradually drain into the suction and discharge pipes. Pipette connections should be cone-shaped to facilitate draining.

8.6. Drain tip

5.6.1. The drain tip must be strong. Glam. conical without sharp narrowing at the drain hole.

$.6.2. The horses of the drain tip must be processed by one of the metols. described below:

a) smooth grinding perpendicular to the axis. slightly beveled outward and melted:

6) smooth ground perpendicular to the axis and slightly bevelled outwards:

c) for pipettes. use for the needs of the national economy. cut and melted.

Fire polishing reduces the risk of breakage during use. but should not cause narrowing. as specified in clause 8.6.1. or morning tension.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to fall from the calibration mark to the drain hole.

The drain time is determined with the pipette in a vertical position, and the receiving vessel should be slightly tilted so. so that the tip of the drain tube is in contact with the inner lining of the vessel. but without movement relative to each other.

Gakim framed. drain time should be in the range. indicated in the table. 4.

Taolina 4

Tree time. ©. Sha pinetek paminalnan imestimimim. mt

Accuracy class

1-n Fet is correct but!

waiting time: not less than Sa not more than 2%

Denmark 15 s: not less than > He boss = 2-H He 40) not more than 29

riznost leysavitsl-

nym and time

no plum!" 2 2 2 3 3 4 4 4 5 5 3

te Esai the time of Sain is indicated on the pipsti in c. 19.2. 1” the actual and indicated values of the time should not differ more than the value indicated in Table 4 Dali pietak. using the needs of the people

9. CALIBRATION MARK

The marking mark must be legible. uniform. indelible line. completely encircling the tube. A break in the mark is allowed, not exceeding 10 “ of the circumference. The line must be flat in the plane. perpendicular to the axis of the pipette. The line thickness should not exceed 0.3 mm. which is provided for by the requirements of clause $.2 of GOST 29044.

Dimensions. determining the position of the graduation mark in the pipette. are listed in Table. 2.

S. 6 GOST 29169-91

10. SIGNS

10.1. All pyrettes must bear the following markings:

a) number. indicating nominal capacity and abbreviation cm? or ml ala notation for units. in which the pipette is graduated (see the note to paragraph 3.1):

6) 20 °C (standard recommended temperature).

Note. There. If the temperature is 27 °C, this value is indicated instead of 20 `C.

c) the letter "Oe", indicating that. that the pipette is designed to dispense the appropriate nominal capacity:

d) designation | 2 to indicate the accuracy class of graded pyrettes.

i) trademark or company name

©) waiting time. if it is installed. in the "0+ 15 s" mode.

10.2. On the pipette class. intended for official verification or certification. Additional markings should be applied. which it is preferable to apply to other pills of the |-th class. and when needed. for pipettes of the 2nd class:

a) the designation of this standard:

6) drain time in seconds.

10.3. Permissible error limit of nominal capacity in accordance with Table. 1 can be applied to all pipettes. e.g. +... ml.

Il. MARKINGS, NUMBERS AND SIGNS

GOST 29169-91
(ISO 648-77)

Group P66

INTERSTATE STANDARD

Laboratory glassware

PIPETTE WITH SINGLE MARK

laboratory glassware. One-mark pipettes

MKS 71.040.20
OKP 43 2133

Introduction date 1994-01-01

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Klin independent design and technology bureau for the design of glass instruments and apparatus

2. APPROVED AND INTRODUCED BY Decree of the Committee for Standardization and Metrology of the USSR dated 12/16/91 N 1952

This standard has been prepared by direct application of the international standard ISO 648-77* "Laboratory glassware - Single mark pipettes" with additional requirements reflecting the needs of the national economy
________________
* Access to international and foreign documents mentioned hereinafter in the text can be obtained by clicking on the link to the site http://shop.cntd.ru. - Database manufacturer's note.

3. INSTEAD OF GOST 20292-74 regarding pipettes with one mark

4. REFERENCE REGULATIONS AND TECHNICAL DOCUMENTS


Number of the section, application in which the link is provided	Designation of the corresponding international standard	The designation of the domestic NTD, to which the link is given
Annex 1
Annex 1
Sections 1, 2, 3, 9; annexes A and B	ISO 384-78

5. REPUBLICATION. March 2008

1. PURPOSE AND SCOPE

This International Standard covers a range of single-mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

GOST 29044-91 (ISO 384-78) Laboratory glassware. Principles of arrangement and design of measuring utensils

3. BASIC UNITS OF MEASUREMENT

3.1. Capacity unit

A cubic centimeter (cm) is taken as a unit of capacity, it is allowed to use the name milliliter (ml).

Note. The term milliliter (mL) is usually used as a special name for the cubic centimeter (cm) according to the International System of Units (SI).

3.2. Standard temperature

Standard temperature, i.e. the temperature at which the nominal volume (nominal capacity) is drained is 20 °C.

Note. When using pipettes in tropical conditions, it is allowed to take not 20 ° C, but 27 ° C as the standard water temperature, as recommended by GOST 29044.

4. VOLUME MEASUREMENT ACCURACY

Two classes of accuracy are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

Pipettes with a nominal capacity of 0.5 ml are made without a reservoir, 1 ml - with and without a reservoir, 2 ml - with a reservoir for the 1st class and with and without a reservoir - for the 2nd class.

All other sizes are for reservoir pipettes.

If necessary, pipettes of all sizes can be made with safety reservoirs above the graduation mark.

Note. Where necessary, pipettes of other nominal capacities may be made provided they conform to the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-marked pipette is defined as the volume of water, expressed in milliliters, at 20 °C, as described below.

Note. If the recommended temperature is 27°C, then this temperature should be set instead of 20°C.

The pipette in the vertical position should be filled with liquid a few millimeters above the graduation mark, and the meniscus drop should then be adjusted to the mark by one of the methods described below:

a) the meniscus is set so that the plane of the upper edge of the calibration mark is horizontal and tangent to the lower point of the meniscus, the observer's gaze is in the same plane;

b) the meniscus is set so that the plane of the center of the mark is horizontal and tangent to the lower point of the meniscus. The observer's gaze is directed upwards to this plane, while the front and rear parts of the mark should be simultaneously visible, simultaneously converging at the bottom of the meniscus.

Then, any droplets adhering to the spout tip at the moment of contact of the spout tip with the surface of the glass vessel are removed.

After that, the liquid must be drained into another, slightly inclined glass vessel so that the drain tip is in contact with the inner wall of the vessel, but without moving them relative to each other during the drain and the waiting period.

The pipette is released until the meniscus drops to the drain hole. To ensure that the drain is complete, wait 3 s before the pipette is removed from the vessel. This applies to pipettes for which no waiting time is set.

Note. The waiting period (approximately 3 s) is set in order to determine the time to drain the liquid. There is no need to strictly adhere to this time. Be sure that the meniscus has reached the draining edge of the pipette before removing the pipette from the receiving vessel.

If the pipette is set to a waiting time of 15 seconds, then this time must be maintained before removing the pipette from the receiving vessel.

Note. Class 1 pipettes must be:

a) without a specified waiting time, when this time is approximately equal to 3 s;

b) with a waiting time of 15 s.

Class 2 pipettes must be without a specified waiting time as defined in subparagraph a.

7. VOLUME ERROR LIMITS

7.1. The limits of permissible error of the nominal capacity should not exceed the values specified in Table 1.

Table 1


Rated capacity	Limit of error
	1st class	2nd grade

8. CONSTRUCTION

8.1. Material

Pipettes with a single mark should be made of glass with adequate chemical and thermal resistance and, as far as possible, free from visible defects and internal stress.

8.2. Form

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and class 2 pipettes with a nominal capacity of 2 ml may be of the design shown in Fig. 1 or may have a reservoir as indicated for large sizes.

Damn.1. Pipette shape

All large size pipettes consist of a reservoir with suction and discharge tubes. These parts must be straight and coaxial.

The shape of the pipettes should correspond to that shown in Figure 1.

If a safety reservoir is provided, it is approximately spherical and is located between the graduation mark and the top of the pipette.

It is allowed for the needs of the national economy to manufacture pipettes with one mark of the following designs:

1 - straight lines (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with an extension and a spare tank (Fig. 2).

Damn.2. Pipette 1-2-50

An example of a symbol for a pipette used for the needs of the national economy, execution 1, 2nd class, with a nominal capacity of 50 ml:

Pipette 1-2-50 GOST 29169-91

8.3. Dimensions

The dimensions of the pipettes should correspond to those indicated in tables 2 and 3.

table 2

Dimensions in mm


Size name	Nominal capacity, ml

Total length, no more than:
straight pipettes
pipettes with reservoir
Suction tube length, not less than
Drain tube length, not less than
Inner diameter at the graduation mark, no more
Outer diameter of drain tube(limit deviation ±1mm)
Tank diameter, no more
Straight pipette tube diameter, no more

For reservoir pipettes only.

For straight pipettes - maximum inner diameter; for reservoir pipettes, the maximum inside diameter of the suction tube.

Estimated minimum distance from the calibration mark to the end of the drain hole, equal to 120 mm (see Table 3).

For pipettes used for the needs of the national economy.

Table 3


Size name	Meaning
Distance of the calibration mark from the top of the pipette, not less than
Distance from the calibration mark to the top of the tank, not less than
Distance from the graduation mark to the cone of the drain hole of straight pipettes, not less than
Pipette wall thickness, not less than:
with a reservoir with a nominal capacity of 1 and 2 ml
other pipettes
Safety tank diameter
Distance from the top of the pipette to the bottom of the safety tank, no more than
Distance from the top of the pipette to the top of the safety reservoir, not less than

Note. Allowed for pipettes used for the needs of the national economy:

1) expansion wall thickness for pipettes with a reservoir - not less than 0.4 mm;

2) the value of the diameter of the safety tank - take as a reference size.

The outer diameter of the discharge tube and the diameter of the straight pipette tube for pipettes used for the needs of the national economy are recommended.

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that might interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or smooth polished with a slight outward bevel.

8.5. Storage tank

The main part of the reservoir is cylindrical, except for pipettes with a nominal capacity of 1 and 2 ml, which may have an oval reservoir if necessary. The two ends of the tank should gradually pass into the suction and drain pipes. Pipette connections should be tapered to facilitate draining.

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp constrictions at the drain hole.

8.6.2. The end of the drain tip must be processed by one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of breakage in use, but must not cause constriction as specified in 8.6.1 or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to fall from the graduation mark to the drain hole.

The drain time is determined with the pipette in a vertical position, and the receiving vessel should be slightly tilted so that the tip of the drain tube is in contact with the inner wall of the vessel, but without moving relative to each other.

Thus, the drain time should be within the limits indicated in Table 4.

Table 4


Accuracy class	Drain time, s, for pipettes with nominal capacity, ml

1st class without defined waiting time:


1st class with a waiting time of 15 s:
at least
no more
2nd class:


Maximum allowable difference between actual and indicated drain time

If the drain time is indicated on the pipette in accordance with clause 10.2, then the actual and indicated time values \u200b\u200bshould not differ by more than the value indicated in Table 4.

For pipettes used for the needs of the national economy.

9. CALIBRATION MARK

10. SIGNS

10.1. All pipettes must bear the following markings:

a) a number indicating the nominal capacity and the abbreviation cm or ml to indicate the units in which the pipette is graduated (see note to 3.1);

c) the letter "O", indicating that the pipette is designed to drain the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

e) waiting time, if set, in the form "0+15 s".

a) the designation of this standard;

b) drain time in seconds.

10.3. The margin of error of the nominal capacity in accordance with Table 1 can be applied to all pipettes, for example ± . . . ml.

11. MARKINGS, NUMBERS AND SIGNS

11.1. All numbers and designations must be of such dimensions and shapes as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and designations must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, then it must be carried out in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A. RELATIONSHIP BETWEEN CAPACITY AND MARGINAL ERROR FOR CLASS 1 PIPETTE

APPENDIX A

RELATION BETWEEN THE CAPACITY AND THE LIMIT OF PERMISSIBLE ERROR FOR PIPETTES OF THE 1st CLASS IN ACCORDANCE WITH THE REQUIREMENTS of clause 4.4 of GOST 29044

Damn.3

APPENDIX B. RELATIONSHIP BETWEEN MARGINAL ERROR AND MENISCA DIAMETER FOR CLASS 1 PIPETTE

APPENDIX B

RELATIONSHIP BETWEEN THE PERMISSIBLE ERRORS AND THE MENISCA DIAMETER FOR CLASS 1 PIPETTE IN ACCORDANCE WITH THE REQUIREMENTS of clause 4.7 of GOST 29044

APPENDIX 1. ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

ANNEX 1

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (clause 8.3) should be checked with a universal measuring tool in accordance with GOST 166 and GOST 427.

13.3. The drain time of pipettes should be checked according to clause 8.7 of this standard.

14. Packing, transportation and storage

14.1. Pipettes are packed in such a way that their safety during transportation and storage is ensured.

14.2. Pipettes packed according to item 14.1 are placed in a shipping container, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

Electronic text of the document
prepared by CJSC "Kodeks" and checked against:
official publication
Glassware and laboratory equipment.
Pipettes. Burettes: Sat. GOSTs. -
M.: Standartinform, 2008

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INTERSTATE STANDARD

LABORATORY GLASS WARE

PIPETTE WITH SINGLE MARK

Official edition

Standartinform

UDC 542.3:006.354

INTERSTATE

Group P66

STANDARD

Laboratory glassware GOST

PIPETTE WITH ONE MARK

laboratory glassware. One-mark pipettes

(ISO 648-77)

MKS 71.040.20 OKP 43 2133

Date of introduction 01.01.94

1. PURPOSE AND SCOPE

This International Standard covers a range of single-mark pipettes for general laboratory use. General requirements - according to GOST 29044.

The requirements of this standard are mandatory.

Additional requirements reflecting the needs of the national economy are given in Appendix 1.

2. LINKS

4. VOLUME MEASUREMENT ACCURACY

Two classes of accuracy are established:

1st - for higher measurement accuracy;

2nd - for less accurate measurements.

5. RANGE OF CAPACITIES

Pipettes with one mark must be manufactured with a nominal capacity of: 0.5; 1; 2; 5; 10; 20; 25; 50; 100 and 200 ml.

For the needs of the national economy, it is allowed to manufacture pipettes with one mark with a nominal capacity of 10.77 ml.

All other sizes are for reservoir pipettes.

Official edition

Reprint prohibited

GOST 29169-91 S. 3

8. CONSTRUCTION

8.1. Material

Pipettes with a single mark should be made of glass with adequate chemical and thermal resistance and, as far as possible, free from visible defects and internal stress.

Pipettes with a nominal capacity of 0.5 ml consist of a straight tube with a drain hole at the lower end.

Pipettes with a nominal capacity of 1 ml and pipettes of the 2nd class with a nominal capacity of 2 ml may be shown in Fig. 1 design or may have a reservoir as indicated for large sizes.

All large size pipettes consist of a reservoir with suction and discharge tubes. These parts must be straight and coaxial.

The shape of the pipettes should correspond to that shown in Fig. 1.

If a safety reservoir is provided, it is approximately spherical and is located between the graduation mark and the top of the pipette.

It is allowed for the needs of the national economy to manufacture pipettes with one mark of the following designs:

1 - straight lines (Fig. 2);

1a - straight lines with a spare tank (Fig. 2);

2 - with extension (Fig. 2);

2a - with extension and spare tank (Fig. 2).

If necessary, pipettes of all sizes can be made with safety reservoirs above the graduation mark.

Note. Where necessary, pipettes of other nominal capacities may be made provided they conform to the essential requirements of this standard.

6. DETERMINATION OF CAPACITY

The capacity of a single-marked pipette is defined as the volume of water, expressed in milliliters, at 20 °C, as described below.

a) the meniscus is set so that the plane of the upper edge of the calibration mark is horizontal and tangent to the lower point of the meniscus, the observer's gaze is in the same plane;

Then, any droplets adhering to the spout tip at the moment of contact of the spout tip with the surface of the glass vessel are removed.

If the pipette is set to a waiting time of 15 seconds, then this time must be maintained before removing the pipette from the receiving vessel.

±0.01

±0.02

±0.1

±0.01

±0.02

±0.1

±0.2

An example of a symbol for a pipette used for the needs of the national economy, execution 1, 2nd class, with a nominal capacity of 50 ml:

8.3. Dimensions

The dimensions of the pipettes must correspond to those indicated in Table. 2 and 3.

Dimensions in mm

table 2

Size name	Nominal capacity, ml
Size name
Total length, no more than: straight pipettes pipettes with reservoir
Suction tube length 1), not less than
Drain tube length 1), not less than
Inner diameter at the calibration mark 2), no more
Drain tube outer diameter 1) (limit deviation ±1 mm)
Tank diameter 1), no more
Straight pipette tube diameter, no more

^ Reservoir pipettes only.

^ For straight pipettes - maximum inner diameter; for reservoir pipettes, the maximum inside diameter of the suction tube.

^ Estimated minimum distance from the calibration mark to the end of the drain hole, equal to 120 mm (see table. 3).

^ For pipettes used for the needs of the national economy.

The outer diameter of the discharge tube and the diameter of the straight pipette tube for pipettes used for the needs of the national economy are recommended.

Table 3

Meaning

Size name

Pipette wall thickness, not less than: with a reservoir with a nominal capacity of 1 and 2 ml of other pipettes

Safety tank diameter

Distance from the top of the pipette to the bottom of the safety reservoir, not more than Distance from the top of the pipette to the top of the safety reservoir, not less than

Note. Allowed for pipettes used for the needs of the national economy:

1) expansion wall thickness for pipettes with a reservoir - not less than 0.4 mm;

2) the value of the diameter of the safety tank - take as a reference size.

GOST 29169-91 S. 5

8.4. The top of the pipette should be perpendicular to the axis of the pipette and free from any defects that might interfere with the establishment of the meniscus.

The ends of the glass tubes should be melted or smooth polished with a slight outward bevel.

8.5. Storage tank

8.6. Drain tip

8.6.1. The drain tip should be strong, smooth, tapered without sharp constrictions at the drain hole.

8.6.2. The end of the drain tip must be processed by one of the methods described below:

a) smoothly ground perpendicular to the axis, slightly beveled outward and melted;

b) smoothly ground perpendicular to the axis and slightly beveled outward;

c) for pipettes used for the needs of the national economy, cut off and melted.

Fire polishing reduces the risk of breakage in use, but should not cause constriction as specified in and. 8.6.1, or internal stress.

8.7. Drain time

The drain time is defined as the time required for the water meniscus to fall from the graduation mark to the drain hole.

Thus, the drain time should be within the limits indicated in Table. 4.

Table 4

Accuracy class		Drain time, s, for pipettes with a nominal capacity,
Accuracy class
1st class without fixed waiting time: at least

1st class with a waiting time of 15 s: not less than

2nd class: no less

The maximum allowable difference between the actual and indicated drain time

^ If the drain time is indicated on the pipette in accordance with and. 10.2, then the actual and indicated time values should not differ by more than the value indicated in Table. 4.

^ For pipettes used for the needs of the national economy.

9. CALIBRATION MARK

The dimensions that determine the position of the graduation mark on the pipette are shown in Table. 2.

10. SIGNS

10.1. All pipettes must bear the following markings:

a) a number indicating the nominal capacity and the abbreviation cm 3 or ml to indicate the units in which the pipette is graduated (see note to 3.1);

Note. Where the standard temperature is 27 °C, indicate this value instead of 20 °C;

c) the letter "O", indicating that the pipette is designed to drain the corresponding nominal capacity;

d) designation 1 or 2 to indicate the accuracy class of graduated pipettes;

e) trademark or name of the manufacturer;

f) waiting time, if set, in the form "0+15 s".

a) the designation of this standard;

b) drain time in seconds.

10.3. Permissible error limit of nominal capacity in accordance with Table. 1 can be applied to all pipettes, eg + ... ml.

11. MARKINGS, NUMBERS AND SIGNS

11.1. All numbers and designations must be of such dimensions and shapes as to be clearly visible under normal conditions of use.

11.2. The graduation mark, numbers and designations must be clearly visible and indelible.

12. COLOR CODING

If color coding is used, then it must be carried out in accordance with the requirements of regulatory and technical documentation approved in the prescribed manner.

APPENDIX A

RELATION BETWEEN THE CAPACITY AND THE LIMIT OF PERMISSIBLE ERROR FOR PIPETTES OF THE 1ST CLASS IN ACCORDANCE WITH THE REQUIREMENTS of clause 4.4 of GOST 29044

ANNEX 1

ADDITIONAL REQUIREMENTS REFLECTING THE NEEDS OF THE NATIONAL ECONOMY

13. Test methods

13.1. The nominal capacity of pipettes should be checked in accordance with the regulatory and technical documentation approved in the prescribed manner.

13.2. The dimensions of the pipettes (i. 8.3) should be checked with a universal measuring tool in accordance with GOST 166 and GOST 427.

13.3. The drain time of the pipettes should be checked by and. 8.7 of this standard.

14. Packing, transportation and storage

14.1. Pipettes are packed in such a way that their safety during transportation and storage is ensured.

14.2. Pipettes packed by and. 14.1 are placed in a shipping container, where they must be protected from movement and damage.

14.3. Pipettes should be transported in clean covered vehicles.

INFORMATION DATA

1. PREPARED AND INTRODUCED by the Elinsky Independent Design and Technology Bureau for the design of glass instruments and apparatus

2. APPROVED AND INTRODUCED BY Decree of the Committee for Standardization and Metrology of the USSR of December 16, 1991 No. 1952

Annex 1

Sec. 1, 2, 3, 9; annexes A and B

5. REPUBLICATION. March 2008